XRN2 Autoregulation and Control of Polycistronic Gene Expresssion in Caenorhabditis elegans
Fig 2
BPNT1 depletion increases xrn-2 mRNA levels without stimulating its promoter activity.
(A, B) wt, bpnt-1(xe22), paxt-1(0), and paxt-1(0); bpnt-1(xe22) animals were cultured from L1 to L4 for 40 hours at 20°C (A) or 30 hours at 26°C (B) before levels of XRN2 and Actin were detected by Western blot. XRN2 levels were normalized to actin levels and shown with values of wt defined as 1.0. Asterisks indicate unspecific bands. (C) Schematic representation of the rpl-43~xrn-2 operon and the qPCR amplicons. Exons are shown as boxes, and qPCR amplicons as thick lines. SL acceptor sites and the 3‘ end cleavage site of rpl-43 are indicated. (D) wt or bpnt-1(xe22) animals were cultured from L1 to L4 for 40 hours at 20°C. Levels of indicated pre-mRNA or mRNA were quantified by RT-qPCR and normalized to act-1 mRNA levels with wild-type values defined as 1 (n = 3, means ± SEM). Values are shown in S1 Table.