A P-Loop NTPase Regulates Quiescent Center Cell Division and Distal Stem Cell Identity through the Regulation of ROS Homeostasis in Arabidopsis Root
Fig 7
SCR and SHR expression are reduced in the absence of APP1.
(A) The reduced expression of SCR and SHR in app1 was confirmed by qRT-PCR analysis. (B) Immunoblotting analysis of SCR in pSCR::SCR-GFP/Col and pSCR::SCR-GFP/app1 transgenes and SHR in pSHR::SHR-GFP/Col and pSHR::SHR-GFP/app1 transgenes. Loading controls in immunoblotting experiments were Rubisco stained with Coomassie Brilliant Blue. (C, D)Expression of the transgenes pSCR::SCR-GFP and pSHR::SHR-GFP were reduced in the app1 mutants. Bar: 50 μm. (E) Quantification of GFP fluorescence shown in (C, D). At least 50 seedlings were examined for each time point for each biological repeat. The data are given in the form of the mean with an associated SD (n = 3); *, P<0.05, Student’s t test.