The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles
Fig 3
eipr-1 acts in head cholinergic neurons to control locomotion.
(A) The eipr-1 cDNA was expressed in an eipr-1(tm4790) mutant background using the following promoters: rab-3 (all neurons, yakSi4 transgene), unc-17 (cholinergic neurons, yakSi5 transgene), unc-17H (a derivative of the unc-17 promoter that lacks the enhancer for ventral cord expression and thus expresses only in head cholinergic neurons [29], yakSi2 transgene), and acr-2 (ventral cord cholinergic motor neurons, yakSi10 transgene). Expression driven by the rab-3, unc-17, and unc-17H promoters rescued the mutant locomotion defect, but expression driven by acr-2 did not (***, P<0.001; ns, not significant, P>0.05). Error bars = SEM; n = 12–20. (B) eipr-1 acts cell autonomously to control dense-core vesicle cargo trafficking. The eipr-1 cDNA was expressed in an eipr-1(tm4790) mutant background using either the rab-3 promoter (all neurons, yakSi4) or unc-129 promoter (a subset of cholinergic motor neurons where NLP-21::Venus is also expressed, yakEx70). Expression driven by both promoters rescued the unc-129p::NLP-21::Venus (nuIs183 transgene) trafficking defect of the mutant (***, P<0.001). Left: representative images. Scale bar: 10 μm. Right: quantification of dorsal cord fluorescence. Error bars = SEM; n = 7–13.