The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles
Fig 2
eipr-1 mutants have defects in trafficking dense-core vesicle cargos.
(A) Left: representative images of NLP-21::Venus (nuIs183 transgene) fluorescence in motor neuron axons of the dorsal nerve cord. Scale bar: 10 μm. Right: quantification of NLP-21::Venus fluorescence levels in the dorsal nerve cord. The mean fluorescence intensity is given in arbitrary units. eipr-1(tm4790), rab-2(nu415), and rund-1(tm3622) mutants have decreased levels of fluorescence in the dorsal cord (DC), indicative of an NLP-21::Venus sorting or trafficking defect (***, P<0.001 compared to wild type). Double mutants of eipr-1(tm4790) with rab-2(nu415) or rund-1(tm3622) are not significantly different than the single mutants (P>0.05). Error bars = SEM; n = 10. (B) FLP-3::Venus (ceIs61 transgene) fluorescence levels in the dorsal nerve cord. ***, P<0.001. Error bars = SEM; n = 12. See S3A Fig for representative images. (C) INS-22::Venus (nuIs195 transgene) fluorescence levels in the dorsal nerve cord. ***, P<0.001. Error bars = SEM; n = 11–12. See S3B Fig for representative images. (D) IDA-1::GFP (ceIs72 transgene) fluorescence levels in the dorsal nerve cord. **, P<0.01. Error bars = SEM; n = 13–14. See S3C Fig for representative images. (E) UNC-17::GFP (oxSi702 transgene) fluorescence levels in the dorsal nerve cord. eipr-1 mutants do not have a defect (P>0.05). Scale bar: 10 μm. Error bars = SEM; n = 16–19. (F) The peptide processing mutant egl-3(ok979) increases the level of NLP-21::Venus (nuIs183 transgene) in the dorsal cord in both wild type and eipr-1(tm4790) mutant backgrounds. Left: representative images. Scale bar: 10 μm. Right: quantification of NLP-21::Venus fluorescence levels in the dorsal nerve cord. ***, P<0.001. Error bars = SEM; n = 9–12.