The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
Fig 9
Dual DenA stability control during fungal development.
The DenA deneddylase supports asexual development. DenA stability is regulated during fungal development by indicated dynamic phosphorylation and dephosphorylation events and the interaction with DipA (blue arrow) or CSN (orange arrow). Phosphorylation of DenA at serine residues S243 and S245 stabilizes the protein during vegetative growth. Stabilization during this growth phase and early stages of development is required for asexual spore formation induced by light. The cytoplasmatic phosphatase DipA controls the interval of septa positioning and is required for asexual development and light dependent inhibition of sexual differentiation. DenA interacts with DipA in the cytoplasm. This complex dynamically shuttles between nuclei and septa. The transition of a stable DenA to an unstable DenA variant coincides with the appearance of C-terminal S253 phosphorylation and dephosphorylation of S243 as well as S245 at later stages of development. Nuclear CSN is required for sexual development. DenA is destabilized by the five CSN subunits in the dashed frame which are localized in the nucleus and might form a common DenA binding surface.