TGFβ and BMP Dependent Cell Fate Changes Due to Loss of Filamin B Produces Disc Degeneration and Progressive Vertebral Fusions
Fig 4
Flnb–/–AF tissues exhibit Collagen X expression.
Left: posterior, Right: anterior. (A, A′, B, B′) RNA in situ staining of P15 T7 IVD sagittal sections. (A, A′) Flnb+/+ and Flnb–/–in situ sections using sense riboprobe as a negative control. (B) Flnb+/+ in situ section using antisense riboprobe against ColX. ColX expression is exhibited in the hypertrophic zone of the Flnb+/+ vertebral body growth plate (black arrows) and not in the IVD. (B′) Flnb–/–in situ section using antisense riboprobe against ColX. ColX expression is exhibited in both the vertebral body growth plate hypertrophic zone (black arrows) as well as the transformed hypertrophic Flnb–/–AF cells (red arrows).