Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects
Fig 3
CENP-ACse4 mislocalizes to regions that are enriched for the histone variant H2A.ZHtz1.
(A) Mean CENP-ACse4 ChIP coverage for the 3Flag-CSE4 (SBY10419, blue), psh1Δ 3Flag-CSE4 (SBY10484, pink), pGAL-3Flag-CSE4 (SBY10425, green) and psh1Δ pGAL-3Flag-CSE4 (SBY10483, orange) strains centered on annotated NDRs [65]. (B) Mean CENP-ACse4 ChIP coverage for the psh1Δ pGAL-3Flag-CSE4 (SBY10483) strain centered on annotated NDRs, binned by NDR length [65]. (C) Mean CENP-ACse4 ChIP coverage for the psh1Δ pGAL-3Flag-CSE4 (SBY10483, orange) strain (left y axis) vs. mean H2A.ZHtz1 ChIP coverage [4] (blue) (right y axis) at all NDRs. (D) Mean CENP-ACse4 ChIP coverage for the psh1Δ pGAL-3Flag-CSE4 (SBY10483, orange) strain (left y axis) vs. mean normalized H2A.ZHtz1 ChIP coverage [4] (blue) (right y axis) at all centromeres. (E) CENP-ACse4 ChIP coverage for the psh1Δ pGAL-3Flag-CSE4 (SBY10483, orange) strain and WT H2A.ZHtz1 coverage [4] (blue) on the chromosome 4 arm between 60,000 bp and 130,000 bp. The scale of the normalized coverage is from 0–20,000 for the CENP-ACse4 ChIP-seq and from 0–301 for the H2A.ZHtz1 ChIP-seq. (F) Overlap between CENP-ACse4 peaks in 3Flag-CSE4 (SBY10419), psh1Δ 3Flag-CSE4 (SBY10484), pGAL-3Flag-CSE4 (SBY10425) or psh1Δ pGAL-3Flag-CSE4 (SBY10483) compared to WT H2A.ZHtz1 nucleosomes [4].