Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage
Fig 7
Localization of Mediator protein to spermatocyte chromatin requires topi function.
(A-H) Indirect immunofluorescence of (A-D) wild type and (E-H) topi-/- spermatocytes stained for (A and E) anti-V5 to detect V5-MED22, (B and F) Fibrillarin, (C and G) DAPI and (D and H) merge, red: V5-MED22, green: Fibrillarin, blue: DAPI. Bars: 10 μm. (I-P) Indirect immunofluorescence of (I-L) wild type and (M-P) topi-/- spermatocytes stained for (I and M) anti-MED26, (J and N) Fibrillarin, (K and O) DAPI and (L and P) merge, red: anti-MED26, green: Fibrillarin, blue: DAPI. Bars: 10 μm. (Q-X) Indirect immunofluorescence of (Q-T) wild type and (U-X) topi-/- spermatocytes stained for (Q and U) anti-HA to setect SA-3HA, (R and V) Fibrillarin, (S and W) DAPI and (T and X) merge, red: SA-3HA, green: Fibrillarin, blue: DAPI. Bars: 10 μm. (Y and Z) live GFP squash showing SA-GFP expression and localization in (Y) wild type and (Z) Med22RNAi testes. (AA-AB”) Indirect immunofluorescence of (AA-AA”) wild type and (AB-AB”) Med22RNAi spermatocytes stained for (AA and AB) Topi, (AA’ and AB’) DAPI and (AA” and AB”) merge, red: Topi, blue: DAPI. Bars: 2 μm.