Molecular Basis of Gene-Gene Interaction: Cyclic Cross-Regulation of Gene Expression and Post-GWAS Gene-Gene Interaction Involved in Atrial Fibrillation
Fig 2
The PITX2c gene positively regulates expression of the ZFHX3 gene. HCT116 cells were transfected with siRNA specific for PITX2c or an expression plasmid for PITX2c and used for isolation of total RNA samples and real time RT-PCR analysis.
A. Real-time RT-PCR analysis for PITX2c. Transfection of siRNA for PITX2c successfully reduced expression of PITX2c. B. Real-time RT-PCR analysis for PITX2c. Transfection of an expression plasmid for PITX2c successfully increased expression of PITX2c. C. Real-time RT-PCR analysis for ZFHX3. Transfection of siRNA for PITX2c reduced expression of ZFHX3. Transfection of an expression plasmid for PITX2c successfully increased expression of ZFHX3. **P<0.01.