Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction
Fig 4
Reduced MRPS34 affects the abundance of mitochondrial respiratory complexes.
BN-PAGE was performed on 75 μg of mitochondria isolated from liver and heart of young (A) and aged (B) Mrps34 wt/wt and Mrps34 mut/mut mice. Mitochondria lysates (75 μg) isolated from liver and heart of Mrps34wt/wt and Mrps34mut/mut young mice (C) and aged mice (D) were analyzed by BN-PAGE and the gels used for immunoblotting. Specific antibodies representing proteins of each of the mitochondrial complexes were used to compare abundance of protein in the wild type and mutant mice. Data are means ± SEM of five separate experiments; *, p < 0.05 compared with control treatments by a 2-tailed paired Student’s t test.