Rrp12 and the Exportin Crm1 Participate in Late Assembly Events in the Nucleolus during 40S Ribosomal Subunit Biogenesis
Figure 4
Rrp12 is required for the export of pre-40S particles out of the nucleus.
(A to G) Top panels, epifluorescence microscopy analysis of the subcellular distribution of GFP-tagged Enp1 (A), Dim1 (B), Tsr1 (C), Pno1 (D), Ltv1 (E), Nob1 (F) and Rio2 (G) in the indicated yeast strains and culture conditions (top). Bottom panels, differential interference contrast (DIC) images of the above preparations. (H) Western blot analysis showing the distribution of Rio2-GFP (top panel) and Pgk1 (bottom panel) in whole cell lysates (W), cytosolic (C) and nuclear (F) fractions obtained from either control rio2-GFP cells (lanes 1 to 3) or GAL::HA-rrp12/rio2-GFP cells growing in galactose-containing medium (lanes 4 to 6) or upon a shift to glucose-containing medium for 9 hours (lanes 7 to 9).