ATPase-Independent Type-III Protein Secretion in Salmonella enterica
Figure 7
FliH functions as a negative regulator of type-III protein translocation.
Export of FlgE-Bla fusion protein into the periplasm was analyzed in various fliHIJ deletion strains. All strains additionally harbored a deletion of the proximal rod genes (ΔflgBC6557) and the FlgE-Bla fusion protein under its native promoter (flgE6569::bla). (A) Minimal inhibitory concentration (MIC) values with flagellar genes expressed at normal levels. (B) Summary of MIC values with flagellar genes expressed at elevated levels due to a PflhD promoter-up mutation (PflhD* = (P1+P4 -10 TATAAT)). The error bars represent the standard error of the mean (SEM) and biological replicates are shown as individual data points.