Role of abd-A and Abd-B in Development of Abdominal Epithelia Breaks Posterior Prevalence Rule
Figure 3
abd-A is required in HNCs for their proliferation.
A) Live cell imaging using esg-Gal4 UAS-GFP shows reduced proliferation of HNCs in the case of abd-A RNAi. B) Immunostaining of 24 h old pupal abdominal epithelial cells in wild type and in the abd-A RNAi with esg-Gal4 show cell type specific loss of abd-A and less number of HNCs in the case of abd-A RNAi (white arrow) in comparison to wild type. The DAPI stained nuclei of LECs (bigger in size) in wild type epithelia are not spherical and show irregular shape indicating HNC induced cell death while in the case of abd-A RNAi these nuclei show round shape. C) Lower panel of 27 h APF abdominal epithelia (placed anterior left) in abd-A RNAi using esg-Gal4 show less number of HNCs (marked by dotted lines) and more number of LECs in comparison to wild type (upper panel). D) Mitotic clones of abd-A mutant are seen in all three abdominal segments. E) The abd-A clones white in color are small without bristles (red dotted lines) while mitotic clones with lighter pigmentation show small bristles (white dotted lines). F) This clone of abd-AD24 is made using stubble (Sb) mutation (gives stubble bristle) on other homologous chromosome. We see a stubble bristle in middle of the clone while normal bristles are mainly present in the light pigmented areas and white patch show rare or no bristles.