Conditional Inactivation of Upstream Binding Factor Reveals Its Epigenetic Functions and the Existence of a Somatic Nucleolar Precursor Body
Figure 5
UBF elimination has only minor effects on global gene expression.
A) Metabolic labeling of Ubffl/fl/Er-cre+/+ and Ubfwt/wt/Er-cre+/+ cells with [3H]-uridine to follow synthesis of U2 and U3 snRNAs, 5.8S rRNA, 5S rRNA, tRNAs and 18S at the given time points pHT. “Bulk 18S” refers to the EtBr input control. The ratio of incorporation into 5.8S relative to 5S (5.8S/5S) is given below each track of the upper panel. B) Boxplot of unbiased expression microarray data for various gene classes. The plots indicate the relative gene expression levels for Ubffl/fl/Er-cre+/+ cells at 72 h pHT relative to levels for Ubfwt/wt/Er-cre+/+ at the same time point. The original data can be found in the GEO databank under the accession number GSE55450.