The POU Factor Ventral Veins Lacking/Drifter Directs the Timing of Metamorphosis through Ecdysteroid and Juvenile Hormone Signaling
Figure 5
Effect of vvl knockdown on genes involved in JH and ecdysone signaling.
(A–C) Effect of knockdown of vvl on the expression of jhamt3 (A), Met (B) and the JH response gene, kr-h1 (C), in fifth instar larvae. Three biological replicates were used per treatment, and each sample was run in triplicates. (D) Effect of methoprene application on the expression of kr-h1 in vvl knockdown larvae. vvl dsRNA-injected larvae were treated with either acetone (control) or methoprene (15 µg) on day 0 of the fifth instar stage. ampr dsRNA-injected animals treated similarly were used as a comparison. Four biological replicates were used per treatment, and each sample was run in triplicates. Means not sharing the same letter are significantly different (p<0.05, ANOVA with Tukey HSD test). (E, F) Effect of knockdown of vvl on the expression of E75 (E) and HR3 (F). (G) Effect of 20E injection on the expression of HR3 in vvl knockdown larvae. Three biological replicates were used per treatment, and each sample was run in triplicates. For (A)–(F), each sample consisted of RNA pooled from five larvae. For (G), each biological replicate consisted of RNA pooled from three larvae. Data are represented as mean +/− SEM.