FAK Acts as a Suppressor of RTK-MAP Kinase Signalling in Drosophila melanogaster Epithelia and Human Cancer Cells
Figure 6
FAK inhibits RTK signalling by impairing Ras/MAPK pathway.
(A–D) Phosphorylated (active) MAPK immunostainings from wing discs with the indicated genotypes. (A–B) When dFAK was expressed in the ptc-compartment (green), pMAPK staining was unchanged compared to GFP-only expressing cells. (C–D) dRETCA expression increased pMAPK staining in the ptc domain but co-expression with dFAK suppressed this dRETCA-induced activation of MAPK. Scale bars, 50 µm. (E) Quantification of pMAPK immunostaining within the ptc stripe (see methods). Intensity of pMAPK signal is represented as relative values to the mean intensity of control tissues (A) (‘ns’: not statistically significant; **** = p<0.0001; n = 4–6 for each genotype).