E3 Ubiquitin Ligase CHIP and NBR1-Mediated Selective Autophagy Protect Additively against Proteotoxicity in Plant Stress Responses
Figure 7
Differential accumulation of heat-induced insoluble proteins in the chip and nbr1 mutants.
The relative abundances of the 10 proteins in the insoluble fraction of heat-stressed wild-type and mutant plants were estimated from their detected peptide numbers after normalizing them to the same amount for total protein (0.1 mg) used for isolation of insoluble proteins. Increased accumulation of an aggregated protein in the mutants after 6 (A) and 9 (B) hours of heat stress was determined by calculating the ratio of the normalized peptide number in the mutants to that in wild type. At1g29910, LHCB1; At2g34420, LHCB1B2; At2g05070, LHCB2; At2g39730, RCA; At5g01530, LHCB4; At1g20620, CAT3; At4g35090, CAT2; At3g62030, ROC4; At4g10340, LHCB5; At4g20360, RABE1B.