A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants Identifies a Novel Factor Involved in Cell Wall Precursor Metabolism
Figure 3
Phenotypes of mutants inactivated for DUF218 factors.
A. Schematic showing the predicted membrane topologies of ElyC and its paralogues SanA and YgjQ. The fourth paralogue, YdcF, is predicted to be cytoplasmic and is not shown. Topology predictions were performed using the TMHMM server (http://www.cbs.dtu.dk/services/TMHMM/). B. CPRG and growth phenotypes of deletion mutants lacking DUF218 factors. Cells of the indicated deletion mutants in an MG1655 strain background were either patched onto CPRG indicator agar (20 µg/ml CPRG and 50 µM IPTG, upper panels) or streaked onto LB agar prepared with 1% NaCl (lower panels). All plates were incubated at room temperature.