Nebula/DSCR1 Upregulation Delays Neurodegeneration and Protects against APP-Induced Axonal Transport Defects by Restoring Calcineurin and GSK-3β Signaling
Figure 6
APP upregulation causes defective transport of synaptotagmin that is restored by Nebula co-upregulation.
(A) Kymographs depicting movement of GFP-SYT. Scale bars: 5 µm (X) and 60 s (Y). Red lines: anterograde movement; blue lines: retrograde movement. (B) Quantification of the total number of moving vesicles, as well as relative movement in the anterograde and retrograde directions normalized to the total number of observed vesicles. APP overexpression (OE) caused significant defects in the movement of GFP-SYT. (C) Speed of GFP-SYT movement in the anterograde and retrograde directions. Values represent mean ± S.E.M, * p≤0.05 compared to control, ** P<0.05 compared to the indicated genotypes. n≥6 independent experiments per genotype.