Genome-Wide Mutation Avalanches Induced in Diploid Yeast Cells by a Base Analog or an APOBEC Deaminase
Figure 4
Dependence of mutational load on ploidy and mutant selection.
A. Number of mutations for different types of HAP-treated genomes. B. Number of mutations for different types of PmCDA1-treated genomes. Bars are median values. Note the logarithmic scale. p values for Mann-Whitney test are shown for significant differences. C. Viability of haploid spores obtained from different diploid clones. Spores resulting from dissecting tetrads produced from wild-type strains (LAN211 and LAN210), mutants induced by HAP (LAN211-5) and PmCDA1 (LAN210-L4), and non-mutant clones treated with HAP (LAN211-NM3) and PmCDA1 (LAN210-NM2) are shown. Each vertical row of four colonies represents the progeny of four haploid spores produced in a single meiosis.