Genome-Wide Identification of Regulatory RNAs in the Human Pathogen Clostridium difficile
Figure 5
Expression analysis and secondary structure prediction of growth phase-regulated sRNAs.
The results are given at the left for RCd6 sRNA and at the right for SQ1498. In corresponding genomic region (A) coding sequences are indicated by blue arrows and new sRNA candidates identified in this study are indicated by grey arrows. For Northern blot analysis (B) RNA samples correspond to those indicated for Figure 2 for 630Δerm strain. 5S RNA at the bottom serves as loading control. The arrows show the detected transcripts with their estimated size. The RNA secondary structure prediction was performed by Mfold software. The loop region of predicted target interaction for RCd6 is highlighted in orange.