Breakpoint Analysis of Transcriptional and Genomic Profiles Uncovers Novel Gene Fusions Spanning Multiple Human Cancer Types
Figure 6
Identification and characterization of FAM133B/CDK6 in J.RT3-T3.5.
(A) Heatmap depicting rearrangement of CDK6 in J.RT3-T3.5 (Jurkat derivative). (B) Discovery of the FAM133B/CDK6 rearrangement by paired-end RNA-seq. The fusion junction was confirmed by RT-PCR (not shown) and Sanger sequencing. (C) Gene expression profiling reveals high-level expression of CDK6 in J.RT3-T3.5 compared to other leukemia cell lines. Note that array probes mapped to the portion of CDK6 retained in the fusion. (D) Jurkat demonstrates marked sensitivity to the CDK4/6 inhibitor PD0332991 (IC50 = 0.27 µM). K562, which expresses only wildtype CDK6, is used as a negative control cell line and shows minimal sensitivity to PD0332991 (IC50 = 5.9 µM).