DNA Topoisomerase III Localizes to Centromeres and Affects Centromeric CENP-A Levels in Fission Yeast
Figure 1
The top3-105 mutant carries an Y209C amino acid substitution and displays impaired growth at 36°C.
(A) Alignment of Top3 amino acid sequences from different species. The position corresponding to Y209 of S. pombe Top3 is highlighted in grey. The amino acids are colored according to their physiochemical properties. An asterisk (*) indicates a fully conserved residue, a colon (:) indicates conservation between groups of strongly similar properties, and a period (.) indicates conservation between groups of weakly similar properties between all species. (B) Spotting of the indicated strains in 5-fold serial dilutions on plates incubated at 25°C, 30°C and 36°C. (C) Growth kinetics of the indicated strains in liquid media after a shift from 25°C to 36°C at time zero.