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Expression of a Novel P22 ORFan Gene Reveals the Phage Carrier State in Salmonella Typhimurium

Figure 7

Evidence for pid transcription during P22 infection at the single cell level.

Phase contrast (left panels) and corresponding YFP epifluorescence (right panels) micrographs of exponential phase cultures of LT2 either (A) 3 h after infection with P22 Δpid::yfp, (B) 3 h after infection with P22 Δint Δpid::yfp, (C) 2 h after infection with P22 c2 Δpid::yfp (just before general cell lysis occurred), or (D) carrying P22 Δpid::yfp as a prophage. Infections with P22 (A, B and C) were performed at MOI = 0.1.

Figure 7

doi: https://doi.org/10.1371/journal.pgen.1003269.g007