Transposon Variants and Their Effects on Gene Expression in Arabidopsis
Figure 4
Relationship of TE siRNA–targeting to distance from genes and its effect on gene expression in Col-0.
(a) Average distance of siRNA− (red) and siRNA+ (yellow) proximal TEs to the nearest gene. For siRNA+ proximal TEs, distance to the closest gene is compared between msiRNA+ TEs (cyan) and usiRNA+ TEs (navy). MWU [siRNA+/siRNA−] p<2.2×10−16 for Col-0, Bur-0 and C24. MWU [msiRNA/usiRNA] p[Col-0]<2×10−16, p[Bur-0] = 6×10−13 and p[C24] = 2×10−6. (b) Average expression level of genes when neighboring TEs are siRNA− (red) or siRNA+ (yellow). For siRNA+ TEs, average gene expression levels are given for when the nearest TE is distal (greater than 2 kb from gene; dark gray) or proximal (within 2 kb; light gray). For genes with proximal siRNA+ TEs, expression levels were further compared between msiRNA+ TEs (cyan) and usiRNA+ TEs (navy). See Figure S12 for Bur-0 and C24. MWU [siRNA+/siRNA−] p[Col-0] = 0.0001, p[Bur-0] = 0.002, p[C24] = 2×10−6. MWU [siRNA+ distal/proximal] p<2×10−16 for Col-0, Bur-0 and C24. MWU [msiRNA/usiRNA] p[Col-0] = 3×10−6, p[Bur-0] = 5×10−5, p[C24] = 0.01). ** = p<0.01. Standard errors are shown.