Importance of Polη for Damage-Induced Cohesion Reveals Differential Regulation of Cohesion Establishment at the Break Site and Genome-Wide
Figure 4
Establishment of DI-cohesion is a function unique for Polη among the TLS polymerases.
(A) Using the Scc1NC system (Figure 1E), establishment of DI-cohesion was compared between WT, rev1Δ, rev3Δ or rad30Δ deletion mutants. One DSB at the MAT locus on Chr. III was induced by pGAL-HO. Percentage of separated sister chromatids were scored in ≥200 cells per time point by the TetR-GFP/Tet-O-system (see Figure 1). (B) Determination of DSB repair during G2 in a triple TLS polymerase deletion strain by PFGE (See Figure 2A for details). (C) Averaged quantifications of pulsed field gels run on the indicated strains (See Figure 2B for details).