Deubiquitylation Machinery Is Required for Embryonic Polarity in Caenorhabditis elegans
Figure 3
PAR-2 function is not required to mediate loss of polarity in math-33(RNAi) usp-47(RNAi).
(A) Embryonic lethality of the indicated genotypes. Error bars indicate the standard error of the mean. The experimental class marked with an asterisk is significantly different from all three controls, p<0.05, by Student's t-test. (B) LGL-1::GFP time-lapse images of embryos of the indicated genotypes. Upon knockdown of the DUBs MATH-33 and USP-47, the LGL-1::GFP domain size is smaller than in control embryos and is not maintained, similar to previous results with PAR-2::GFP in Figure 2C, and transverse spindles occur in P1. Scale bar represents 5 µm.