Genetic Inhibition of Solute-Linked Carrier 39 Family Transporter 1 Ameliorates Aβ Pathology in a Drosophila Model of Alzheimer's Disease
Figure 7
dZip1 overexpression results in Zn accumulation in Aβ42-expressing fly brains revealed by Zinquin staining.
10-day old flies were treated with zinc-rich food (4 mM ZnCl2) for 40 h and 72 h, respectively). Dissected brains were treated with 25 mM Zinquin for 30 min and then examined with a fluorescent microscope. No fluorescent signal was detected without Zinquin treatment (A–B). dZip1 OE (elav-Gal4>UAS-Aβ42/UAS-dZip1, E–F) led to an obvious Zn accumulation in the neocortex and neuropile region of the fly brains as compared with the control Aβ42 flies (elav-Gal4>UAS-Aβ42, C–D). Weaker signal was detected when dZip1 was knocked down by RNAi (elav-Gal4>UAS-Aβ42/UAS-dZip1-RNAi 1#, G–H). elav-Gal4 fly brains showed similar Zinquin signals as dZip1-RNAi fly brains (I–J). Relative Zinquin signals against background in the neocotex and neuropile region were quantitated using ImagJ software (K). The relative intensity of Zinquin signal, however, is by no menas a quantitative reflection of zinc levels [42]. Data are expressed as means ± SEM and analyzed by Student's t-test. n = 4 for each genotype. Scale bar, 100 µm.