Transcriptional Control in Cardiac Progenitors: Tbx1 Interacts with the BAF Chromatin Remodeling Complex and Regulates Wnt5a
Figure 9
Tbx1 interacts with Baf60a in mouse embryo tissues.
(A) Co-IP experiment showing interaction of the Tbx1 and Baf60a endogenous proteins in embryo tissues. The first lane on the left contains nuclear extracts from E9.5 WT embryos. The second and third lanes contains nuclear extracts from E9.5 Tbx1−/− and WT embryos immunoprecipitated with an anti-Baf60a antibody. Western blot with an anti-Tbx1 antibody clearly show co-immunoprecipitation in the WT sample. (B) Whole mount in situ hybridization with an anti-sense and sense (control) Baf60a probe. Note a nearly ubiquitous hybridization signal but with very little or no expression in the heart.