Computational Prediction and Molecular Characterization of an Oomycete Effector and the Cognate Arabidopsis Resistance Gene
Figure 3
ATR39 alleles are expressed in different Hpa isolates, but expression of ATR39-1 does not correlate with avirulence on Wei-0.
A) Amino acid sequence alignment of ATR39-1 and ATR39-2 using ClustalW. B) RT-PCR with allele-specific primers from plants infected with the indicated Hpa isolates, harvested at 7 dpi. Sequencing of the amplified ATR39 fragments revealed the absence of additional polymorphisms in Hpa strains. C) Trypan blue staining of Wei-0 leaves inoculated with the indicated Hpa strains. Presence or absence of ATR39 alleles is indicated below, as well as qualitative scoring of Hpa growth on Wei-0. C, cotyledons; T, true leaves; S, susceptible; R, resistant.