An RNA Transport System in Candida albicans Regulates Hyphal Morphology and Invasive Growth
Figure 2
She3-associated transcripts accumulate in yeast buds and in hyphal tips.
Cells from wild type (“WT,” CAF2-1) and she3Δ/she3Δ (SE4) strains were processed for FISH to detect endogenous She3-associated transcripts; cell nuclei were visualized with DAPI. Representative examples illustrate She3-dependent localization of the indicated transcripts. (A) Probe signal accumulates in the incipient bud (MSS4), or bud (CDC20, CHT2) of wild type C. albicans yeast. There is no specific localization of probe signal in yeast cells lacking She3. (B) A control ACT1 probe is not localized in yeast cells from either strain. (C) In wild type hyphae, probe signal accumulates in the distal end of the germ tube (SAP5) or hyphal tip cell (RBT4, orf19.1536). Hyphae shown hybridized with SAP5, RBT4, and orf19.1536 probes were collected, respectively, 30 minutes, one hour, or three hours after serum induction. As in yeast, probe signals are not localized in hyphae lacking She3. (D) A control probe for ACT1 is not localized in germ tubes from either strain collected 40 min after serum induction. Signals from two additional control probes, ACC1 and ADH1, were not localized in yeast or hyphae (data not shown).