Sfrp Controls Apicobasal Polarity and Oriented Cell Division in Developing Gut Epithelium
Figure 5
Defective epithelial AB polarity in Sfrps-deficient small intestine.
(A) Histological (H&E) staining revealed a clump of epithelial cells in the Sfrp1−/− Sfrp2−/− and Sfrp1−/− Sfrp2−/− Sfrp5+/− small intestine at E13.5 (arrowhead). Scale bar: 50 µm. (B–G) Epithelial AB polarity is disrupted in the Sfrp1−/− Sfrp2−/− Sfrp5+/− small intestine (C, E, G) in comparison to the control small intestine (B, D, F), as evidenced by the sub-cellular distribution patterns of activated aPKC (B–G in green), F-actin (B, C in red), ß1-integrin (D, E in red) and E-cadherin (F, G in red). Scale bar: 25 µm.