Gγ1, a Downstream Target for the hmgcr-Isoprenoid Biosynthetic Pathway, Is Required for Releasing the Hedgehog Ligand and Directing Germ Cell Migration
Figure 2
Reduced wingless expression in embryos compromised for gγ1.
Panels A and B: Embryos from gγ1N159/ Cy0, en:LacZ stock were collected and fixed using standard procedures. Embryos were genotyped by probing with β-galactosidase antibodies (imaged in red: not shown), while Wg accumulation was visualized by probing with Wg (imaged in green) antibodies. Embryos carrying en:LacZ express β-galactosidase whereas homozygous mutant embryos do not. The embryo in panel A was positive for β-galactosidase (not shown) and has at least one wild type copy of gγ1. Note the high level of Wg accumulation in the stripes. The embryo in panel B β-galactosidase negative, and is homozygous for the gγ1N159 mutation. Note the lower level of Wg expression. Panels C and D: Embryos from a gγ1k0817/Cyo, en:LacZ stock were collected and fixed using standard procedures. As in panels A and B embryos were genotyped by probing with β-galactosidase antibodies (imaged in red: not shown), while Wg accumulation was visualized by probing with Wg (imaged in green) antibodies. The embryo in C is positive for β-galactosidase, while the embryo in D is not. Note the difference in Wg accumulation in the blow-up of three Wg stripes.