A Motor Function for the DEAD-Box RNA Helicase, Gemin3, in Drosophila
Figure 6
Gemin3 disruption in mesoderm and larval muscles has a drastic impact on adult viability.
Bar charts showing adult fly viability assayed at 25°C (A) and 29°C (B). Fly viability is unaffected when the gemin3ΔN transgene is driven in post-mitotic neuronal tissues via elav-GAL4, nrv2-GAL4, D42-GAL4 and OK6-GAL4. Lethality is however obvious when Gemin3 is disrupted in all tissues via Act5C-GAL4 or in mesoderm and larval muscles through expression by how-GAL4 and C179-GAL4. A significant reduction in viability was also observed when the gemin3ΔN was driven in the muscles via G7-GAL4 and at 29°C via C57-GAL4. When highly expressed at 29°C, mef2-GAL4, which expresses in mesoderm and larval muscles, also has a significant effect on viability. Driver-associated lethality was rescued on co-expression of a full-length gemin3 transgene. Individual bars represent the mean viability ± 1.0 S.E.M. of 4 independent experiments. The ♦ and ⋄ symbols indicate lethality.