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The HLH-6 Transcription Factor Regulates C. elegans Pharyngeal Gland Development and Function

Figure 6

Phenotypic analysis of hlh-6 mutants.

(A–B) The stuffed pharynx phenotype of hlh-6 mutants grown on OP50-GFP bacteria. (A) NDIC image, (B) merged NDIC and fluorescence image. Anterior is at left and scale bars represent 10 µm. (C–E) Assays for growth defects in wild-type, hlh-6 mutants and hlh-6 mutants rescued by either the hlh-6 genomic fragment, the hlh-6 minigene or by using the HB101 strain of E. coli. (C) Graph of body length versus time, (D) time to reach adulthood and (E) brood sizes. For the hlh-6 mutants the L1 arrested animals are omitted. Error bars represent one standard deviation.

Figure 6

doi: https://doi.org/10.1371/journal.pgen.1000222.g006