No Influence of Indy on Lifespan in Drosophila after Correction for Genetic and Cytoplasmic Background Effects
Figure 4
Successive Backcrossing Abolishes Indy206-Associated Longevity
A) Survival of the original versus wDah-backcrossed (×8) males on SY food. The data are male progeny derived from crosses between Indy206 (or +/+) homozygote mothers and +/+ males. Median lifespan is 68 and 46 d for CS-Indy206/+ and CS (+/+) males, respectively (Log rank test χ2 = 131.17, p < 0.0001), and 55 and 53 d for wDah-Indy206/+ and wDah (+/+) males, respectively (Log rank test χ2 = 0.06, p = 0.8009).
B) Survival of the wDah-backcrossed (×10) males on SY food. The flies are all progeny from crosses between wDah-Indy206/+ females and wDah-Indy206/+ males. Median lifespans are 48, 48, and 50 d for homozygous, heterozygous and +/+ males, respectively. Log-Rank test χ2 and p-values: wDah-Indy206/+ versus wDah-Indy206/ Indy206 (χ2 = 1.34, p = 0.2467), wDah-Indy206/+ versus +/+ (χ2 = 2.08, p = 0.1493), and wDah-Indy206/ Indy206 versus +/+ (χ2 = 7.37, p = 0.0066).
C) Survival of wDah-backcrossed (×10) females on SY food. The flies are all progeny from the same crosses as males in Figure 3B. Median lifespans are 54, 54 and 54 d for homozygous, heterozygous and +/+ females, respectively. Log-Rank test χ2 and p-values: wDah-Indy206/+ versus wDah-Indy206/ Indy206 (χ2 = 1.37, p = 0.2423), wDah-Indy206/+ versus +/+ (χ2 = 0.63, p = 0.4272), and wDah-Indy206/ Indy206 versus +/+ (χ2 = 0.00, p = 0.9980).