Dynamic modelling of the PI3K/MTOR signalling network uncovers biphasic dependence of mTORC1 activity on the mTORC2 subunit SIN1
Fig 2
Independent validation of the four models.
Simulations of the dynamic response of various network components to insulin stimulation in control (WT MEF cells, blue lines) condition and when TRAF2 is deleted (TRAF2-/- MEF cells, red lines), in comparison to the corresponding experimental data (right panels), taken and quantified from [7]. The simulation data are presented as mean ± S.E.M. Experimental data points were reproduced from representative data of at least three biological replicates obtained from [7]. These validation analysis indicates models 1–3 could not recapitulate all data, and model 4 represents the best-fit model (see text for details).