Predicting proteome allocation, overflow metabolism, and metal requirements in a model acetogen
Fig 4
Predicted and experimental gene expression.
Categorized by RAST subsystem and summed, predicted gene expression (transcription flux reactions * gDW of RNA molecule; mmol* h-1) was compared to RNA-seq data for C. ljungdahlii grown on (A) CO, (B) CO2+H2, and (C) fructose. Linear regressions, 95% confidence intervals of the regression, and 95% prediction intervals are represented by lines, dark shaded areas, and light shaded areas respectively. Scatter plots shown are for the highest Pearson r between predicted and experimental data. Normalized total transcription flux (mmol*gDW-1*h-1) of the Wood-Ljungdahl pathway was plotted against carbon substrate uptake rate for (D) CO, (E) CO2+H2, and (F) fructose. Pearson r reflects correlation with growth rate.