Whole genomes define concordance of matched primary, xenograft, and organoid models of pancreas cancer
Fig 5
Comparative analysis of structural variation (SV) across tumour-PDX pairs of the primary and metastasis cohorts.
(A) Distribution of SV events (deletion, duplication, inversion, translocation) in each sample across 10 primary-PDX pairs (left) and 6 metastasis-PDX pairs (right). (B) Chromosome-specific Jaccard indices across 10 primary-PDX pairs (left) and 6 metastasis-PDX pairs (right). Samples are labeled by their PCSI identifier. Chromosomes with an observed large number of rearrangements (≥5 events) in both the tumour and matched PDX are indicated (black stars). Chromosomes with a large difference of SV events between a tumour-PDX pair (≥10 SV events difference) are highlighted (red stars). The overall concordance (Sc) score for a tumour-PDX pair across all chromosomes in indicated (bottom row). (C) Genome-wide SV events observed in tumour-PDX pairs in PCSI_0169 (resected primary, left) and PCSI_0491 (liver metastasis, right). Each type of SV event is color-coded with a similar color between tumours and matching PDX. For each SV type, tumours are annotated on the outer rings of the circos plot and the matching PDX on the inner rings. Chromosomes exhibiting clustered SV events (potential chromothripsis) are highlighted in the blue boxes. (D) Comparison of SV events across chr18-chr22 for the PCSI_0611 primary tumour and its matching PDX. There is an apparent chromothripsis event on chr22 of the PDX but not the primary sample.