The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects
Fig 2
Correlation between Tm values of sequence subsections within siRNA duplexes and the corresponding off-target silencing efficiency.
The start of the subsection within a duplex is plotted on the Y axis (‘Start Position’) whereas the end of the subsection is plotted on the X axis (‘End Position’). The position numbering mirrors that used in Fig 1. The analysis was performed separately for each siRNA concentration–(A) 0.05, (B) 0.5, (C) 5 and (D) 50 nM. The siRNA sequences used in the analysis, together with corresponding knockdown percentages, are listed in S1 Table.