The Effects of Alignment Quality, Distance Calculation Method, Sequence Filtering, and Region on the Analysis of 16S rRNA Gene-Based Studies
Figure 4
The Morisita-Horn coefficient calculated between two mock communities (described in Materials & Methods) using different OTU definitions and alignments.
Each bar represents the average coefficient value for 100 randomized partitionings of the data. Within the same OTU cutoff, alignment strategies with the same symbol and regions with the same letter were not significantly different from each other.