SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-α autoinhibition
Fig 6
Constitutive activation of SYD-2 leads to precocious aggregation and degradation.
(A) HSN synapse formation phenotypes visualized with Airyscan superresolution imaging of endogenous mScarlet-SYD-2 and indicated mutants. SAM1-3Δ and 3E mutants are depleted at HSN synapses. Scale bar, 5 μm. (B) Quantification of HSN intensities in (A). (C) HSN cell body imaging of endogenous mScarlet-SYD-2 and indicated mutants; 10 μm of the proteasome inhibitor bortezomib or DMSO as a control was added to the indicated animals for 4 h prior to imaging. Scale bar, 5 μm. (D) Quantification of SYD-2 puncta in (C). ****, p < 0.001. (D) Quantification of total SYD-2 cell body intensity in (C). ****, p < 0.001. Underlying data is available in S1 Data. HSN, hermaphrodite-specific neuron.