Construction of an artificial phosphoketolase pathway that efficiently catabolizes multiple carbon sources to acetyl-CoA
Fig 2
The distribution and new functions of PKs.
(A) The distribution of PKs across the tree of life, as referenced in Jillian F. Banfield’s study [38]. The blue color in pie chart represents species with PKs present in all genome sequenced species. (B) The catalytic activity of PKs from different species on different substrates. The displayed 7 PKs not only exhibited activity on F6P or Xu5P, but also demonstrated the ability to convert short-chain ketoses into AcP. The corresponding table on the right represents the catalytic activity of these 7 candidate PKs on 6 classes of ketose or ketose phosphate. Each color represents to a specific enzyme activity (U/mg). Detailed catalytic activity data are shown in S1 Table. The raw data was listed in S1 Data. AcP, acetyl-phosphate; F6P, fructose-6-phosphate; PK, phosphoketolase; Xu5P, xylulose-5-phosphate.