PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites
Fig 3
PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are essential for distal MTOC vesicle localization during neurites outgrowth.
(A) Kymographs of the growing PVD anterior dendrite expressing EBP-2::mKate2 (PVD specific) and GIP-2::GFP (knock-in). Scale, 5 μm. (B, C) Colocalization of GIP-2::GFP (knock-in) and mKate2::RAB-11 (PVD expressed) in the distal segment of the growing PVD anterior dendrite. Line scans for intensity profile of each channel from distal dendrites to cell body is shown in (C). Scale, 5 μm. PVD neurons are indicated with red dashed lines in (B). (D) Vesicle dynamics of PVD neurons expressing GFP::RAB-11 in the distal segment of the growing anterior dendrite in wild-type and indicated mutants. White arrowheads point to the dynamic RAB-11 clusters. Scale, 5 μm. Dashed line marks the developing dendrite. (E) GIP-2::GFP (knock-in) localization in the wild-type and indicated mutants; green: GIP-2, magenta: fill of PVD neurons. GIP-2 cluster are indicated with blue arrowheads, and the PVD neurons are indicated with red dashed lines. Scale, 5 μm. (F, G) Quantification of RAB-11 (D) and GIP-2 (E) localization in wild-type and indicated mutants in the developing PVD neuron; light gray: no GIP-2 cluster was observed, dark gray: RAB-11 or GIP2 cluster localized in cell body, black: RAB-11 or GIP-2 accumulated in the distal developing dendrites. Number of analyzed animals is indicated. (H, I) Quantification of number of GIP-2::GFP puncta (H) and line scans of the average intensity from dendritic tip to cell body in the distal segment of the growing PVD anterior dendrite (I).Error bars represent SD; statistical analysis, Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Number of analyzed animals is indicated. The data underlying the graphs shown in the figure can be found in S1 Data.