Step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo
Fig 4
The left plot shows the coordinates of tomogram ts001 (z-view) visualized with the Dynamo command dtplot using the resulting table from the first alignment. The coordinates moved from their initial positions (compare Fig 2) and adapted to the shape of the VLPs. The cropped subvolumes are large enough to cover all the empty spaces between the coordinates. This is visualized in the plot on the right where disks with a diameter matching the side length of the subvolumes (256 pixels) are placed at each coordinate and successfully cover the whole surface of the VLPs (visualized using the Dynamo command dpktbl.plots.disks). The data and commands to exactly reproduce this figure are available on EMPIAR (EMPIAR-10702). EMPIAR, Electron Microscopy Public Image Archive; VLP, virus-like particle.