Drosophila-associated bacteria differentially shape the nutritional requirements of their host during juvenile growth
Fig 4
ApWJL and LpNC8 can differentially fulfill their host’s nutritional requirements in HDs.
(A) Heat map representing the mean D50 of GF larvae (first column) and larvae associated with ApWJL, LpNC8, ApWJLHK, and LpNC8HK (columns 2, 3, 4, 5 respectively). Each line shows D50 in a different version of HD: complete HD (first line) or HDs lacking nutrient X (ΔX, lines below). White means larvae did not reach pupariation in these conditions. Means, standard errors of the mean and statistical tests (Dunn test of multiple comparisons) are detailed in S4 Table. (B–D) Absence of correlation between time of development and quantity of bacteria. Y axis shows D50, and X axis shows quantity of bacteria (Log10 CFUs) in the larval gut (B), in the diet in presence of larvae 3 days after inoculation (C), and in the diet in presence of larvae 6 days after inoculation (D). Each dot shows a different condition. Complete HD: on complete HD. ΔX: on HDs lacking nutrient X. Black dots: in monoassociation with ApWJL, green dots: in monoassociation with LpNC8. For each bacterium, we tested Pearson’s product–moment correlation between D50 and quantity of bacteria. ApWJL, A. pomorumWJL; CFU, colony-forming unit; cor, Pearson correlation coefficient for each bacterium; D50, day when 50% of larvae population has entered metamorphosis; EAAFly, fly essential amino acid; GF, germ-free; HD, Holidic Diet; HK, heat-killed; LpNC8, L. plantarumNC8; NALs, nucleic acids and lipids; NEAAFly, fly nonessential amino acid.