Gasdermin D mediates the pathogenesis of neonatal-onset multisystem inflammatory disease in mice
Fig 3
GSDMD deficiency prevents the onset of systemic inflammation in NOMID mice.
(A) IL-1β levels in mouse bone marrow supernatants. (B) WBC counts. (C) Neutrophils. (D) RBCs. (E) Flow cytometry analysis of bone marrow cells stained with antibodies against CD11b, Gr-1, or Ter119. (F) Quantitative data from flow cytometry dot plots shown in panel E. Data are mean ± SEM. Three-week-old mice were used for the studies. WT mice (panel A: 2 males and 1 female; panel B and C: 3 males and 2 females; panel D: 5 males and 4 females; panel F: 2 males and 1 female for CD11b+/Gr-1+, and 3 males and 1 female for Ter119+). NOMID mice (panel A: 2 males and 1 female; panel B and C: 4 males and 3 females; panel D: 4 males and 3 females; panel F: 2 males and 1 female). NOMID;Gsdmd−/− mice (panel A: 2 males and 1 female; panel B–F: 2 males and 1 female); Gsdmd−/− mice: (panel A: 2 males and 1 female; panel B and C: 3 males and 3 females; panel D: 4 males and 4 females; panel F: 3 males and 1 female). The numerical values underlying Fig 3A, 3B, 3C, 3D and 3F can be found in S1 Data. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001. GSDMD, gasdermin D; IL-1β, interleukin-1β; NOMID, neonatal-onset multisystem inflammatory disease; RBC, red blood cell; WBC, white blood cell; WT, wild-type.