Virus-Mediated Alterations in miRNA Factors and Degradation of Viral miRNAs by MCPIP1
Fig 3
KSHV infection affects expression of miRNA biogenesis machinery.
(A) HUVECs were treated with cytokines: IL-1β, TNFα, and TWEAK. Total RNA was harvested at 2 or 24 h after treatment, and MCPIP1 expression was measured using qPCR. Results are shown relative to β-actin and normalized to the untreated control. N = 5–11 per group. (B) Host mRNA expression data in KSHV latently infected HUVECs (48 hpi) [20]. Expression changes of Dicer, MCPIP1, TARBP1, and TARBP2 are indicated. (C) Western blots for MCPIP1 protein are shown for HUVECs that were either mock-infected or infected with KSHV, N = 3. For all graphs, results are shown as mean ± SD. Significance was assessed using a Student’s t test, *p ≤ 0.05, **p ≤ 0.01. Numerical data can be found in S1 Data.