Tor1 and CK2 kinases control a switch between alternative ribosome biogenesis pathways in a growth-dependent manner
Fig 3
TOR pathway controls the switch between the A2 and A3 pathways.
(A) Pre-rRNA processing in YMK118 grown in YPD (OD600 = 1.7) and treated with either DMSO or rapamycin, analyzed by northern blotting using probe A2–A3. (B) A rapamycin-insensitive tor1-1 strain was treated the same as in (A). (C) Comparison of iBAQ values (normalized to bait) of 350 proteins detected in the affinity-purified preribosomes from cultures treated with DMSO or rapamycin. Proteins changed more than 2-fold are in black. (D) Comparison of SILAC H/L ratios (normalized to bait) of proteins from the affinity-purified preribosomes affected either by diauxic shift or by rapamycin treatment. (E) Histogram of SILAC H/L ratios (normalized to bait) for proteins with more than 2-fold change in the preribosomes purified from rapamycin versus DMSO-treated cultures. An experiment with a higher coverage is shown. Underlying numerical data are in S1 Data.