Calorie Restriction-Mediated Replicative Lifespan Extension in Yeast Is Non-Cell Autonomous
Figure 4
A low molecular weight factor in CR-conditioned media is required for the longevity benefit of CR.
(A) RLS analysis for wild-type BY4741 in dialyzed 2% glucose and glucose-restricted conditions indicates that the longevity factor is a small molecule. (B) Percent increases in median life span relative to RLS on 2% glucose without migration. Data were collected from four independent experiments with n = 30. ns, no significant difference; *, p < 0.05; ***, p < 0.001 in comparison to the 2% not moved group.